Planning permission has been given for the new enterprise on Vansittart Industrial Estate.
Co-founder Willie Calvert said: "As a Windsor resident myself, we are delighted to have received the go-ahead for our new brewery to be within sight of the castle.
"We think our beer will prove to be a big hit for locals and many of the 17million visitors who come to Windsor and Eton each year."
He added: "It's a tough time for the on-trade, as you can see from pubs closing here in Windsor, but we can help to lift the market - because you have to go out to drink great cask-conditioned beer.
"Local breweries are good news for local people, local pubs and local
The last brewery in Windsor, Burge & Co based in Victoria Street, closed in 1931.
The new Windsor and Eton Brewery will be set up in a 3,900 square foot unit and is expected to open in April using British designed and built brewing vessels.
The brewers are aiming to produce 30 barrels a week, with each barrel containing 36 gallons.
Another of the brewery's founders is Master Brewer Paddy Johnson. He said: "When I was Head Brewer at Courage there were perhaps just 50 breweries in the country - now there's over 700. That's fantastic for drinkers. But there's still not a brewery for the people of Windsor, Eton and Slough - until now. We're looking forward to serving you!"
The unit will be transformed by ex-Mars engineering manager Jim Morrison who will be overseeing the installation of the plant. For more information visit www.webrew.co.uk